You must perform a "kill curve" for your specific cell line and G418 batch prior to the experiment.
Provides resistance to G418 (Geneticin), an antibiotic that usually kills mammalian cells. 🚀 Key Applications
When a PGK-Neo cassette is inserted into a gene locus, the strong PGK promoter can influence the expression of neighboring genes. It might drive the expression of an upstream or downstream exon, or it might alter the chromatin state of the region. This can complicate the interpretation of phenotypic data. If a scientist knocks out a gene to see what happens, but the
The "gold standard" for creating knockout mouse models.
It acts as a "motor" that drives the constant expression of the resistance gene. It is a strong, constitutive promoter
